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Both xCT inhibition and transsulfuration blockade suppress intraocular tumor growth in an orthotopic MYCN -amplified RB model. ( A ) Experimental timeline showing intravitreal injection of <t>luciferase-labeled</t> Y79 cells on day 1; model validation on day 7; intravitreal injection of PBS, IKE, PAG, or IKE+PAG on days 11, 15, and 19; and eyeball collection on day 22. ( B ) Representative bioluminescence images on days 7, 11, 15, and 19 for each group. ( C ) Quantification of bioluminescence signal, body weight curves, and eyeball weight at day 22. ( D ) Representative H&E, Ki67, and 4-HNE immunohistochemical staining of ocular sections from each group, together with quantitative analysis of 4-HNE–positive areas. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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Both xCT inhibition and transsulfuration blockade suppress intraocular tumor growth in an orthotopic MYCN -amplified RB model. ( A ) Experimental timeline showing intravitreal injection of luciferase-labeled Y79 cells on day 1; model validation on day 7; intravitreal injection of PBS, IKE, PAG, or IKE+PAG on days 11, 15, and 19; and eyeball collection on day 22. ( B ) Representative bioluminescence images on days 7, 11, 15, and 19 for each group. ( C ) Quantification of bioluminescence signal, body weight curves, and eyeball weight at day 22. ( D ) Representative H&E, Ki67, and 4-HNE immunohistochemical staining of ocular sections from each group, together with quantitative analysis of 4-HNE–positive areas. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: Investigative Ophthalmology & Visual Science

Article Title: M YCN Amplification Drives Ferroptosis Susceptibility via Cysteine Metabolism in Retinoblastoma

doi: 10.1167/iovs.67.5.9

Figure Lengend Snippet: Both xCT inhibition and transsulfuration blockade suppress intraocular tumor growth in an orthotopic MYCN -amplified RB model. ( A ) Experimental timeline showing intravitreal injection of luciferase-labeled Y79 cells on day 1; model validation on day 7; intravitreal injection of PBS, IKE, PAG, or IKE+PAG on days 11, 15, and 19; and eyeball collection on day 22. ( B ) Representative bioluminescence images on days 7, 11, 15, and 19 for each group. ( C ) Quantification of bioluminescence signal, body weight curves, and eyeball weight at day 22. ( D ) Representative H&E, Ki67, and 4-HNE immunohistochemical staining of ocular sections from each group, together with quantitative analysis of 4-HNE–positive areas. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: To generate luciferase-expressing retinoblastoma cells, Y79 cells were transduced with a self-inactivating lentiviral system carrying the luciferase gene (Shanghai Genechem Co., Ltd.).

Techniques: Inhibition, Amplification, Injection, Luciferase, Labeling, Biomarker Discovery, Immunohistochemical staining, Staining